Application

Research CategoryStem Cell Research

This Murine Embryonic Stem Cell line is derived from C57BL/6N blastocysts.

Cell Line Description

Embryonic Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Embryonic Stem Cell line derived from C57BL/6N blastocysts. C57BL/6N is the most commonly used inbred mouse strain. Large scale mouse knockout projects such as NIH’s KOMP project use C57BL/6 as the standard background for mouse genome engineering. C57BL/6 is also the reference strain for mouse genome sequencing at the NHGRI. C57BL/6N mice show good breeding performance. As a commonly used reference strain, transgenic mouse models are often backcrossed to a uniform C57BL/6 background, and B6 ES cells facilitate building complex models which combine gene inactivation with existing transgenics maintained in a B6 background. Additional phenotypic information is available at the Mouse Genome Informatics website (http://www.informatics.jax.org/external/festing/mouse/docs/C57BL.shtml). The B6 ES line was genome verified by determining the alleles at 29 SNPs that are informative across a large number of inbred mouse strains.

STRAIN: C57BL/6N

GENDER: Male

KARYOTYPE: 40XY

Legal Information

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

2.5 x 10⁶ cells per vial, passage 9

Physical form

Cells are supplied frozen in basal medium supplemented with 50% FCS and 10% DMSO

Storage and Stability

Place vials in liquid nitrogen immediately upon receipt until it is convenient to proceed to subculture.